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. 2011 Apr 28;7(4):e1002058. doi: 10.1371/journal.pgen.1002058

Figure 4. The set of CBK1-interacting genes includes transcriptional targets of Ace2.

Figure 4

The binding of Ace2-TAP to the promoter regions of 5 CBK1-interacting genes was assessed by ChIP in yeast and hyphae-phase cells (SM, 3 h, 37°C) containing a TAP-tag fused to the C-terminus of Ace2. Bars indicate the ratio of promoter DNA (determined by PCR) in tagged extracts relative to un-tagged extracts (error bars indicate SD of three replicates). Grey bars show promoters with increased abundance in tagged extracts, suggesting they are bound by Ace2. P cht3, a known target of Ace2 [13], [21], and primers to a coding sequence (ORF cds) serve as positive and negative controls. A persistent contaminating band prevented accurate assessment of ACT1 in hyphae.