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. 2011 Apr 28;7(4):e1002058. doi: 10.1371/journal.pgen.1002058

Figure 6. Elevated PKA activity accounts for increase pseudohyphae in RAM mutants.

Figure 6

(A) ace2Δ/Δ cells were incubated in YPD at 30°C for 3 h −/+ PKA inhibitor MyrPKI (10 µM) and examined by light microscopy. (B) EFG1 expression was determined by semi-quantitative RT-PCR for each strain at the indicated time after transfer to SM at 37°C. ACT1 levels were used as loading control. The graph indicates the fold change in EFG1 levels for the mutant strains relative to wild type at the 180 min time point. The bars indicate the mean fold change in EFG1 relative to wild type for three independent replicates and the error bars indicate standard deviation. The brackets indicate that the difference between EFG1 transcript levels was statistically significant for each mutant relative to wild type (Student's t test, p<0.02). (C) The expression of ENO1 and PGK1 were examined in the indicated strains as described in Figure 5A. The brackets indicate that the difference between PGK1 transcript levels was statistically significant for the two mutants (Student's t test, p<0.02).