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. 2011 Apr 28;6(4):e19010. doi: 10.1371/journal.pone.0019010

Table 2. Effect of repeated sampling on molecular detection of parasite clones and on multiplicity of infection.

P. falciparum P. vivax P. vivax P. vivax
msp2 msp1F3 MS16 2 markers combined1
Detection of parasite clones
No. clones detected only on day 1 93 (18.0%) 382 (23.8%) 495 (25.2%)
No. clones detected only on day 2 90 (17.4%) 307 (19.2%) 617 (31.3%)
No. clones detected on both days 335 (64.7%) 912 (57.0%) 855 (43.5%)
Total No. of clones 518 1601 1967
Detectability Inline graphic [95% CI] 0.79 [0.76–0.82]2 0.73 [0.71–0.75]2 0.61 [0.58–0.63]2
Multiplicity of infection
MOI on day 1 1.53 2.31 2.34 2.78
MOI on day 2 1.47 2.11 2.52 2.77
MOI on both days 1.68 2.60 3.10 3.37
1

The highest value for MOI of either marker msp1F3 or MS16 was used.

2

Detectability refers to individual genotypes at day 1 versus day 2.