Table 2. Effect of repeated sampling on molecular detection of parasite clones and on multiplicity of infection.
P. falciparum | P. vivax | P. vivax | P. vivax | |
msp2 | msp1F3 | MS16 | 2 markers combined1 | |
Detection of parasite clones | ||||
No. clones detected only on day 1 | 93 (18.0%) | 382 (23.8%) | 495 (25.2%) | |
No. clones detected only on day 2 | 90 (17.4%) | 307 (19.2%) | 617 (31.3%) | |
No. clones detected on both days | 335 (64.7%) | 912 (57.0%) | 855 (43.5%) | |
Total No. of clones | 518 | 1601 | 1967 | |
Detectability [95% CI] | 0.79 [0.76–0.82]2 | 0.73 [0.71–0.75]2 | 0.61 [0.58–0.63]2 | |
Multiplicity of infection | ||||
MOI on day 1 | 1.53 | 2.31 | 2.34 | 2.78 |
MOI on day 2 | 1.47 | 2.11 | 2.52 | 2.77 |
MOI on both days | 1.68 | 2.60 | 3.10 | 3.37 |
The highest value for MOI of either marker msp1F3 or MS16 was used.
Detectability refers to individual genotypes at day 1 versus day 2.