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. 2011 Apr 28;6(4):e19000. doi: 10.1371/journal.pone.0019000

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Bone marrow macrophages were either left untreated as a control (open bars) or treated with 10 mM MβCD for 1 h (solid bars) prior to infection. Separate experiments were performed for wild-type and LcJ amastigotes as well as for non-opsonized and 5% A/J serum opsonized parasites. Parasite load and intracellular survival was quantified as described. Data indicate parasites per 100 macrophages for wild-type (A and B) and LcJ (C and D) amastigotes and are the means ± SE of 3 repeats in triplicates (non-opsonized) or duplicate conditions (serum opsonized). Statistical analysis, (t-test), * P = <0.05, ** P = <0.001.