Table 2. Summary of all data generated by behavioral observations and the pup retrieval test.
Balb/c GR +/+ | Balb/c GR +/− | C57Bl/6N GR +/+ | C57Bl/6N GR +/− | Transf | Strain | Genotype | Interaction | ||
In nest | |||||||||
licking/grooming [%] | 7,951±1,070 | 9,790±1,454 | 16,615±1,273 | 11,101±2,476 | NT | 0,008 ** | 0,043 * | ||
active nursing [%] | 14,613±4,955 | 9,913±3,589 | 6,130±2,323 | 11,800±3,654 | sqrt | ||||
passive nursing [%] | 43,471±4,482 | 49,027±3,801 | 64,870±4,993 | 57,299±7,275 | NT | 0,013 * | |||
nest building [%] | 6,624±1,089 | 6,058±1,317 | 5,532±0,857 | 10,346±3,220 | sqrt | ||||
self-grooming [%] | 4,517±0,579 | 6,140±1,710 | 3,848±0,863 | 5,304±1,214 | sqrt | ||||
pups out of nest [%] | 5,507±2,484 | 2,070±1,485 | 1,498±1,265 | 0,246±0,123 | sqrt | 0,086 T | |||
Out of nest | |||||||||
eating/drinking [%] | 10,387±2,299 | 10,835±1,664 | 14,422±3,166 | 10,067±3,808 | NT | ||||
self-grooming [%] | 1,783±0,410 | 2,908±1,037 | 1,010±0,338 | 0,360±0,198 | sqrt | 0,002 ** | |||
climbing/digging [%] | 6,924±1,514 | 5,578±1,186 | 1,718±1,254 | 2,477±1,123 | NT | 0,004 ** | |||
Pup retrieval test | |||||||||
handling [%] | 26,410±4,661 | 24,658±5,226 | 34,614±8,825 | 47,402±6,456 | NT | 0,048 * | |||
pups back in nest [s] | 165,286±35,313 | 135,600±35,730 | 107,167±33,491 | 83,000±22,290 | angular | ||||
crouching over pups [s] | 232,714±33,622 | 166,600±39,829 | 178,500±41,680 | 206,143±44,680 | angular | 0,051 T |
Data analysis was done using GLMs on the basis of 26 dams belonging to four different treatment groups: Balb/c wildtypes (+/+; n = 7), Balb/c with a heterozygous mutation of the GR (+/−; n = 6 for behavioral observations, n = 5 for the pup retrieval test) C57BL/6N wildtypes (+/+; n = 6), C57BL/6N with a heterozygous mutation of the GR (+/−; n = 7). Data are given as untransformed means ± standard error of the mean (s.e.m.). The statistical analysis is summarized with respect to the transformation used (NT = not transformed, sqrt = square root transformation, angular = angular transformation) and the effects of “strain”, “GR genotype” and “strain-by-GR-genotype-interaction” (T = tendency; *p<0.05, **p<0.01).