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. 2011 Apr 28;6(4):e19190. doi: 10.1371/journal.pone.0019190

Figure 2. SSL5 activates human platelets and induces syk and src dependent platelet aggregation.

Figure 2

(A) Levels of anti-P-selectin mAb or (B) PAC-1 binding were measured by flow cytometry in samples of washed platelets after incubation with PBS, 20 µM ADP, 100 ng/ml convulxin, or 30 µg/ml SSL5 or T175P SSL5. (C) Representative aggregation trace obtained in washed platelets, induced by 20 µg/ml SSL5 or no stimulation (PBS). Aggregation was strongly inhibited by pre-treatment of platelet with either a syk kinase inhibitor (BAY61-3606, 5 µM), or a src inhibitor (PP2, 3 µM). Images are representative of three independent experiments. * P<0.05.