Table 2. Dissociation constants of human ODC-AZ and AZI-AZ complexes.
[ODC-AZ] complex | K d,ODC-AZ (µM) | [AZI-AZ] complex | K d,AZI-AZ (µM) |
ODC_WT | 0.21±0.001 | AZI_WT | 0.02±0.009 |
ODC_N125K | 0.10±0.001 | AZI_K125N | 0.16±0.009 |
ODC_M140K | 0.14±0.001 | AZI_K140M | 0.11±0.001 |
ODC_N125K/M140K | 0.02±0.002 | AZI_K125N/K140M | 0.18±0.01 |
The dissociation constants (K d) of ODC-AZ and AZI-AZ were derived from the global fitting of the sedimentation velocity data to the model of A+B↔AB hetero-association in the SEDPHAT program.