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. 2011 Apr 28;6(4):e19342. doi: 10.1371/journal.pone.0019342

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Colonques et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Confocal projection taken through a late larval brain hemisphere from a ventro-anterior orientation showing PROS mRNA expression in numerous scattered cells in the OPC and CB, GRH expression, and counterstaining with Propidium Iodide (PI). A1. High magnification view of the cell cluster framed in A showing a single PROS mRNA expressing cell (small arrow) located in close vicinity to a large NB (arrowhead) and a GMC (empty arrowhead), both expressing GRH. A2. Schematic representation of the cellular expression pattern of pros. B. Similar confocal projection as in A showing PROS mRNA and MIR expression. B1. High magnification of the CB cell cluster framed in B showing a single PROS mRNA expressing cell located near a large MIR+ NB. C. Confocal projection over 30 µm taken from a frontal orientation of the OPC. Notice the presence of several PROS mRNA expressing cells located underneath the layer of MIR+ cells.