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. Author manuscript; available in PMC: 2012 May 5.
Published in final edited form as: DNA Repair (Amst). 2011 Apr 3;10(5):536–544. doi: 10.1016/j.dnarep.2011.03.001

Fig. 6.

Fig. 6

Increased loss of the I-SceI site during NHEJ of DSBs near telomeres. The frequency of loss of the I-SceI site during NHEJ between the two I-SceI sites in the pEJ5-GFP plasmid was compared for cell clones containing interstitial and telomeric integration sites. (A) The pEJ5-GFP plasmid integrated at telomeric (EJ5-6D, EJ5-6F, EJ5-6J) or interstitial (EJ5-7F, EJ5-AB, EJ5-AE) sites were used to determine the relative percentage of loss and restoration of the I-SceI site. Genomic DNA from pooled populations of cells infected with the pQCXIH-ISceI retrovirus and selected with hygromycin for 10 days was amplified by PCR using oligonucleotide primers distal to the two I-SceI sites in pEJ5-GFP (small black arrows). (B) The PCR products were then digested with I-SceI endonuclease. (C) The percentage of cut and uncut DNA was determined from the intensity of the bands using Image J. Experiments were performed in triplicate and standard deviations are shown.