TABLE 3.
Mobility parameters in the OHC lateral plasma membrane after cholesterol manipulations
| Depleted | Control | Loaded | ||||
|---|---|---|---|---|---|---|
| Whole cochlea | ||||||
| 0.5 mM (n = 10) | (n = 17) | 0.1 mM (n = 8) | 0.5 mM (n = 11) | |||
| Mf (%) | 90 ± 6 | 94 ± 5 | 93 ± 7 | 88 ± 5a | ||
| D (μm2/s) | 1.08 ± 0.60 | 1.67 ± 0.89 | 1.31 ± 0.47 | 0.81 ± 0.42a | ||
| R2 | 0.84 ± 0.10 | 0.83 ± 0.09 | 0.87 ± 0.10 | 0.87 ± 0.08 | ||
| Isolated OHCs | ||||||
| 0.1 mM (n = 5) | 0.05 mM (n = 8) | 0.05 mM (n = 8) | 0.1 mM (n = 4) | 1 mM (n = 6) | ||
| Mf (%) | 86 ± 7a | 94 ± 3 | 94 ± 5 | 91 ± 6 | 92 ± 5 | 87 ± 6 |
| D (μm2/s) | 0.54 ± 0.18a | 1.27 ± 0.32 | 1.67 ± 0.89 | 0.70 ± 0.35a | 0.72 ± 0.32a | 0.29 ± 0.08a |
| R2 | 0.95 ± 0.02 | 0.83 ± 0.07 | 0.83 ± 0.07 | 0.91 ± 0.04 | 0.94 ± 0.02 | 0.97 ± 0.02 |
When examining the mobile fraction, only 0.5-mM loading of whole cochlea and 0.1-mM depletion of isolated OHCs showed reduction in Mf values that were different from control (p < 0.05). However, various loading and depletion treatments showed significant decreases in D values compared to control (p < 0.05). Values are shown as mean ± SD. R2 values demonstrating goodness of fit for calculations of D are also provided
aStatistically significant differences from control based on one-factor ANOVA with Dunnett’s post hoc testing