TABLE 4.

Effect of cholesterol manipulations on lateral mobility parameters in HEK cells

	Depleted 10 mM (n = 21)	Depleted 5 mM (n = 17)	Control (n = 17)	Loaded 5 mM (n = 16)	Loaded 10 mM (n = 24)
Mf (%)	86 ± 5	86 ± 6	88 ± 6	87 ± 9	86 ± 7
D (μm2/s)	0.82 ± 0.36	0.70 ± 0.26	0.81 ± 0.30	0.96 ± 0.57	1.01 ± 0.42
R2	0.90 ± 0.07	0.94 ± 0.04	0.94 ± 0.03	0.91 ± 0.09	0.89 ± 0.04

Membrane cholesterol manipulations do not produce significant changes to quantitative lateral mobility parameters in HEK cells (p > 0.1). Values shown are mean ± SD. R² values demonstrating goodness of fit for calculations of D are also provided