FIG. 3.

Treatment with VPA does not protect hair cells from streptomycin ototoxicity. Organotypic cultures of the chick utricle were used to determine whether VPA treatment reduced the ototoxic effects of streptomycin. Utricles were cultured for 48 h in A control medium, B 24 h in 1 mM streptomycin followed by 24 h in drug-free control medium, or C 24 h in 1 mM streptomycin and 1 mM VPA followed by 24 h with 1 mM VPA alone. Utricles were then fixed and surviving hair cells were labeled with the HCS-1 antibody. Co-treatment with VPA did not appear to prevent hair cell death following streptomycin exposure. Scale bar 25 μm.