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Expression of HDAC1 and HDAC2 in the sensory epithelium of the chick utricle. Cultures of dissociated supporting cells were prepared and mRNA extracted with TRIzol. Reverse-transcription polymerase chain reaction was performed, using primers for HDAC1 (lane 2) and HDAC2 (lane 4). The expected fragment size was amplified for both targets. Lanes 1 and 6 contain 1-kb ladder, lanes 3 and 5 are HDAC1 and HDAC2 negative controls, respectively.
