FIG. 7.

HDAC1, 2, and 3 inhibition causes decreased proliferation. A Dissociated supporting cell cultures were treated for 24 h with various doses of MS-275, an HDAC 1, 2, and 3 specific inhibitor. We observed that MS-275 treatment caused a dose-dependent decrease in proliferation. B Dissociated cultures treated with increasing doses of TSA. A comparable decrease of proliferation occurred with both 50- and 100-nM doses. C Dissociated cultures were treated with either TSA (100 nM), MS-275 (5 μm), or both drugs, and proliferation indices were calculated. No change in proliferation occurred between treated groups. Error bars: standard deviation, *p < 0.05.