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. 2011 Apr 15;352(2-10):341–352. doi: 10.1016/j.ydbio.2011.02.002

Fig. 5.

Fig. 5

Misexpression of Lrnn1 across posterior midbrain and anterior hindbrain allows cells to move across the MHB. (A) Schematic representation of electroporation followed immediately by iontophoresis of Dil label (x) at 6/8th of (y), the distance along the neural tube from diencephalon to hindbrain constriction (just anterior to Otx2:Gbx2 interface) at HH9. (B,G) Position of Dil label at Ohr. (B–F) GFP control; dorsal view. (C) Neural tube 24 h later. (D) Cells labelled with Dil after 24 h are clustered in posterior midbrain and do not cross the MHB boundary. (E) Control GFP expressing cells are continuous throughout posterior midbrain, MHB and anterior hindbrain, (F) Merged view of Dil labelled cells and GFP expressing cells. (G–K) Lrrnl-IRESGFP; dorsal view. (H) Neural tube 24 h later. (I) Cells labelled with Dil are observed extensively in both midbrain and hindbrain compartments 24 h after labelling. (J) Lrnn1-IRESGFP expressing cells are seen in both midbrain and crossing the MHB into hind-brain. (K) Merged view shows Dil labelled cells within the Lrrnl-electroporated domain in both midbrain and hindbrain. MHB: midbrain–hindbrain boundary; black arrows indicate position of Dil label.