Table 1.
PCR Primer sequences and reaction conditions used for the amplification of sequences encoding Plasmodium ovale sp. tryptophan-rich antigen (PoTRA) and P. ovale reticulocyte-binding protein homologue (PoRBP2).
| Gene | Primer name | Primer sequence | PCR product size (bp) | PCR cycling conditions (Thermocycler) |
|---|---|---|---|---|
| potra | PoTRA fwd3 PoTRA rev3 |
5′-GCACAAAAATGGTGCTAACC-3′ 5′-ATCCATTTACCTTCCATTGC-3′ |
787 | 95 °C for 2 min; (95 °C for 30 s; 58 °C for 45 s; 72 °C for 1 min) × 44 cycles; 72 °C for 5 min (TETRAD) |
| PoTRA fwd5 PoTRA rev5 |
5′-ACGGCAAACCCGATAAACAC-3′ 5′-GTGTTTGTAGTATTTACAGG-3′ |
245 to 355 |
95 °C for 2 min; (95 °C for 30 s; 52 °C for 45 s; 68 °C for 1 min) × 44 cycles; 68 °C for 5 min (TETRAD) | |
| porbp2 | Porbp2TMfwd Porbp2TMrev |
5′-TTGCAAACAAAAGTGCTCC-3′ 5′-CCTAATTCTCTTTGT(G/A)CCC-3′ |
120 | 95 °C for 15 min; (95 °C for 15 s; 53 °C for 30 s; 68 °C for 30 s) × 40 cycles; melt 55 to 95 °C, 0.5 °C steps, 5 s per step. (RG3000/RG6000) |