Fig. 2.

Monitoring correct dosing using BPI. Mice were inoculated with luminescent Citrobacter rodentium ICC180 via the intraperitoneal route (a, b) and by oral gavage (c, d), and the success of the administration was determined by BPI. The animals in (a) and (c) have been correctly dosed with administration of the inocula delivered into the peritoneum (a) and gastrointestinal tract (c). The animals in panels (b) and (d) have been incorrectly dosed, with the inocula being inadvertently delivered into the bladder (b) and lungs (d). Images were acquired using an IVIS system (Caliper Life Sciences) with an integration time of 1 min and are displayed as pseudocolour images of peak bioluminescence, with variations in colour representing light intensity at a given location. Red represents the most intense light emission, while blue corresponds to the weakest signal. The colour bar indicates relative signal intensity (as photons s⁻¹ cm⁻² sr⁻¹).