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. Author manuscript; available in PMC: 2011 Oct 28.
Published in final edited form as: Nature. 2011 Apr 28;472(7344):471–475. doi: 10.1038/nature10071

Figure 1. Particulate, but not soluble, β-glucans induce Dectin-1 signalling.

Figure 1

a Size (molecular weight or diameter) of β-glucan preparations used in this study. b-e Bone marrow-derived macrophages (bmM; b-d IFN-γ-primed overnight) were stimulated with 50 μg/ml β-glucans. b TNF-α production (24 h) was assessed by ELISA; data are means plus standard deviations of triplicate culture (*** p<0.001, n.s. not significant). c ROS production was assessed by luminol-ECL; datapoints are means of triplicate culture. d Syk activation (10 min) was assessed by intracellular flow cytometry. e p38 MAP kinase activation at the indicated times was assessed by immunoblotting. All data are representative of at least 3 independent experiments.