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. Author manuscript; available in PMC: 2011 Oct 28.
Published in final edited form as: Nature. 2011 Apr 28;472(7344):471–475. doi: 10.1038/nature10071

Figure 2. Immobilised β-glucans induce Dectin-1 signalling.

Figure 2

a Soluble β-glucan binding (50 μg/ml, 10 min) to wild type and Dectin-1 -/- bone marrow-derived DC (bmDC) was assessed by flow cytometry. b, c IFN-γ-primed bmM were stimulated with soluble β-glucans (50 μg/ml) or β-glucans immobilised on either tissue culture plates (b – plate-coated) or 0.8 μm polystyrene latex beads (c – beads). ROS production was measured by luminol-ECL; datapoints are means of triplicate culture. d, e TNF-α production (24 h) by bmDC exposed to soluble/particulate (50 μg/ml), plate-immobilised or bead-coated soluble β-glucans was assessed by ELISA; data are expressed as means plus standard deviations of triplicate culture (*** p<0.001, n.s. not significant). All data are representative of at least 3 independent experiments.