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. Author manuscript; available in PMC: 2011 Oct 28.
Published in final edited form as: Nature. 2011 Apr 28;472(7344):471–475. doi: 10.1038/nature10071

Figure 3. CD45 and CD148 regulate Dectin-1 signalling.

Figure 3

Responses of wild type and CD45/CD148-deficient bmM (b IFN-γ-primed) were examined. a Internalization of FITC-labelled zymosan particles (20 μg/ml, 10 min) was assessed by flow cytometry. b ROS production was measured by luminol-ECL; datapoints are means of triplicate culture. c TNF-α production (50 μg/ml WGP, 24 h) was assessed by ELISA; data are expressed as means plus standard deviations of triplicate culture (* p<0.01, *** p<0.001, n.s. not significant). d Syk activation (pSyk; green) by AlexaFluor647-labelled WGP (20 μg/ml, 1 min; blue) was assessed by confocal microscopy. e Inactive Lyn (pY507) levels following zymosan/WGP stimulation (50 μg/ml, 10 min) were assessed by immunoblotting. All data are representative of 3 independent experiments.