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. 2011 Mar;79(6):1574–1593. doi: 10.1111/j.1365-2958.2011.07542.x

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Copyright © 2011 Blackwell Publishing Ltd

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Fig. 3 — Inactivation or downregulation of polyubiquitin affects C. albicans cell morphology.A. Light microscopy of C. albicans ubi4/ubi4 cells grown in YPD at 30°C reveals morphological abnormalities: UBI4/UBI4 (BWP17); ubi4/ubi4 (MLC40) (Table 1).B. Downregulation of UBI4 in C. albicans MET3p-UBI4/ubi4 cells with 2.5 mM methionine and cysteine (Met/Cys) essentially phenocopies the ubi4/ubi4 null mutants: UBI4/UBI4 (BWP17); MET3p-UBI4/ubi4 (MLC05).C. Calcofluor White staining of C. albicans ubi4/ubi4 cells reveals true hypha formation in some cells: UBI4/UBI4 (BWP17); UBI4/ubi4 (MLC35); ubi4/ubi4 (MLC40).Images were taken at the same magnification and with the same exposures.