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. 2011 Mar;79(6):1574–1593. doi: 10.1111/j.1365-2958.2011.07542.x

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Copyright © 2011 Blackwell Publishing Ltd

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Fig. 4 — Inactivation of polyubiquitin affects C. albicans nuclear segregation.A. DAPI staining of C. albicans ubi4/ubi4 cells grown in YPD at 30°C reveals defects in mitosis and aberrant nuclear segregation in some cells: UBI4/UBI4 (BWP17); UBI4/ubi4 (MLC35); ubi4/ubi4 (MLC40).B. DAPI and Calcofluor White staining of ubi4/ubi4 cells under hypha-inducing conditions highlights the extent to which nuclear segregation is incomplete: UBI4/UBI4 (BWP17); UBI4/ubi4 (MLC35); ubi4/ubi4 (MLC40).C. Percentage of cells with aberrant nuclear segregation: UBI4/UBI4 (BWP17); UBI4/ubi4 (MLC35); ubi4/ubi4 (MLC40). **P < 0.01.