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. Author manuscript; available in PMC: 2011 Apr 29.
Published in final edited form as: Oncogene. 2009 Feb 9;28(12):1485–1495. doi: 10.1038/onc.2008.459

Figure 3.

Figure 3

Ras-induced hyperplasia is Mek gene dosage-dependent. Whereas the presence of two Mek alleles is sufficient for full hyperplasia, the presence of one Mek allele is associated with reduced hyperplasia, and no hyperplasia is observed in skin with no Mek alleles. (a) Histology and immunofluorescence staining of adult mouse skin after 5 days of treatment with 4-hydroxytamoxifen (4OHT). Tissue genotypes are noted at the top of each column and markers stained are noted to the right. Scale bars = 50 μm. (b) Quantification of hyperplasia. The interfollicular epidermis was measured using a micrometer. Error bars represent the mean ± SEM (n = 5 per genotype). The difference in skin thickness between wild-type (WT) skin versus ER:Ras induction in 2–4 allele Mek skin (*, p < 2×10−5) and WT skin versus ER:Ras induction in 1 allele Mek skin (**, p < 0.0008) are statistically significant by Student's t test analysis.