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. Author manuscript; available in PMC: 2011 Apr 29.
Published in final edited form as: Oncogene. 2009 Feb 9;28(12):1485–1495. doi: 10.1038/onc.2008.459

Figure 4.

Figure 4

Changes in Ras effector pathways following ablation of Mek1/2 alleles. (a) Epidermal extracts of the noted genotype, after treatment with either 4OHT or ethanol diluent control, were probed with the antibodies indicated at right. (b) GTP-bound Ral recovered with GST-RalBD (upper panel) and Ral present in the epidermal mouse extract or EGF-stimulated normal human keratinocytes (NHK) (lower panel) were identified by Western analysis using a RalA specific antibody. (c) Immunohistochemistry of adult mouse skin after 5 days of treatment with 4OHT or ethanol. The genotypes are noted on top, the antibodies used at right. Scale bar = 50 μm.