Multidose studies. A, endogenous CTL function. Top, experimental design. Bottom, CTL function in B10.D2 (left) and ProHA × TRAMP (right) animals. Three animals per group, representative of two experiments. B, cyclophosphamide + T-GVAX in multidose regimen. HA-specific CD8+ T cells were adoptively transferred on day −2 to ProHA × TRAMP mice. Prostate glands were harvested on day +13 after initial treatment and evaluated for IFN-γ secretion by intracellular staining. Three animals per group, representative of two experiments. C, representative dot plots for panel B.