Skip to main content
. 2011 May;13(5):428–438. doi: 10.1593/neo.101620

Figure 1.

Figure 1

CaR mRNA and protein expression in lung SCC cells and the effect of [Ca2+]o on PTHrP secretion. (A) RT-PCR amplification of CaR from HEK293T (HEK) (historical negative control for CaR expression) (lane 2), BEN, RWGT2 (RW), and HARA (HA) lines and positive controls (+Cntrls) CaR-transfected HEK293T (HEK-CaR) (lower lane 2) human kidney tissue (KID), the PC-3 prostate carcinoma cells (PC-3), and the breast cancer line, MDA-MB-231(MDA). Lane 1 is a ladder. CaR-specific human primers amplified a single 481-bp product in all cell lines including HEK293T. (B) Relative CaR expression by Western blots of cell extracts from adult human kidney (KID) (+ control) lane 1, HEK293T cells (HEK) from ATCC lane 2, and lung SCCs. Experiments were repeated three times and data from a representative blot are shown. (C–E) PTHrP secretion after treatment with increasing [Ca2+] or spermine (2.0 mM). CaR activation increased [PTHrP] at all time points with an ECmax of approximately 3.0 mM Ca2+ excluding spermine treatments. Each value is the mean ± SD of three experiments. *Significant difference between treatment and cells grown in 0.15 mM (*P < .05 vs 0.15 mM).