Figure 2.

Increasing [Ca²⁺]_o induced a biphasic Ca²⁺_i rise in lung SCC cells. (A–D) The graphs are representative of fluo-3 fluorescent Ca²⁺_i responses observed in typical experiments using each lung SCC line and HEK293T cells. Ca²⁺_i baseline was measured in 0.0 mM Ca²⁺ solution, and Ca²⁺_i oscillations were initiated by the addition of 0.15 mM Ca²⁺. The panels below are confocal microscopy images depicting fluo-3-loaded cells at baseline (a) and subsequent emittance of fluorescence on stimulation with Ca²⁺_o (b). Magnification, x10.