Figure 6.

Ca²⁺_o-stimulated Ca²⁺_i release in RWGT2 or RWGT2 transduced with CaR^WT, CaR^A986S, CaR^Q1011E, or CaR^R990G. (A–E) The graphs are representative of rhod-2 fluorescent Ca²⁺_i oscillations observed in RWGT2 and RWGT2 lines stably transduced with a GFP-expressing construct for either CaR wild-type receptor (CaR^WT) or the CaR mutations, CaR^A986S, CaR^Q1011E, or CaR^R990G. Ca²⁺_i baseline was measured in 0.0 mM Ca²⁺ bathing solution, and Ca²⁺_i oscillations were initiated by the addition of 0.15 mM Ca²⁺. The panels below the Ca²⁺_i response curves labeled (a, b) are confocal microscopy images depicting rhod-2-loaded cells at baseline (a) and subsequent emittance of fluorescence on stimulation with Ca²⁺_o (b). The third panel in B to E depicts GFP expression from a GFP-CaR fusion protein.