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. Author manuscript; available in PMC: 2011 Apr 29.
Published in final edited form as: Protein Expr Purif. 2008 Dec 10;65(1):66–76. doi: 10.1016/j.pep.2008.11.013

Fig. 3.

Fig. 3

Initial transcription by S. oneidensis RNAP involves scrunching of promoter DNA. (A) Derivative of lacCONS promoter utilized to monitor the contraction of DNA between positions −15 (Cy3B, as donor) and +15 (Alexa647, as acceptor). The promoter −35 and −10 elements are grey-colored, as is the start site, which is boxed and indicated with an arrow. (B) Left, schematic of DNA scrunching by RNAP. Right, two representative E* histograms compromising of the free DNA (lowest E* value, solid black line), RPo (dashed black line) or RPitc,≤ 7 (higher E* values, corresponding to DNA bending by the RNAP, solid red line).