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. Author manuscript; available in PMC: 2011 Apr 29.
Published in final edited form as: Protein Expr Purif. 2008 Dec 10;65(1):66–76. doi: 10.1016/j.pep.2008.11.013

Fig. 5.

Fig. 5

Kinetics of σ release during early and mature elongation. (A) Fractional occupancy θ (t) for RPo on lacCONS-11 template DNA. Five independent measurements are fitted with a single-exponential (solid line) and errors bar reflect the standard error of mean (SEM). (B) σ70 retention in early elongation complex, RDe,11. The same as (A), with the y intercept corresponding to 5 min after the addition of the NTP subset (ATP, GTP, UTP) to RPo. (B) Early elongation complex with filled RNA exit channel, RDe,14. (C) Mature elongation complex, RDe,50.