. Author manuscript; available in PMC: 2011 Apr 29.

Published in final edited form as: Protein Expr Purif. 2008 Dec 10;65(1):66–76. doi: 10.1016/j.pep.2008.11.013

Table 1.

Plasmids for the polycistronic overexpression plasmid pYK201, and the σ subunits.

Plasmid	Description	His₆ tag position
pSH101	α + C-terminal His₆ tag + PacI inserted between NdeI and NcoI of pET-22b(+)	C
pSH102	β + SacI inserted between NcoI and NdeI of pET-16b
pSH103	β′ + NotI inserted between NcoI and NdeI of pET-16b, changed start codon from GTG to ATG
pSH104	ω + NotI inserted between NcoI and NdeI of pET-16b
pSH202	rbs site + β from pSH102 inserted between PacI and SacI of pSH101
pSH203	rbs site + β′ + FseI from pSH103 inserted between SacI and NotI of pSH202
pYK201	rbs site + ω of pSH104 inserted between FseI and NotI of pSH203	C (of α)
pSH001	σ⁷⁰ inserted between NheI and XhoI of pTrcHis A	N
pNG045	σ⁷⁰ inserted between NheI and EcoRI of pET-28b	N
pNG201	σ^70ΔCys inserted between EcoRI and NotI of pET-DUET	N
pNG203	σ^{70, N399C} inserted between EcoRI and NotI of pET-DUET	N
pNG079	σ³⁸ inserted between NcoI and EcoRI of pETM-11	N
pNG080	σ³⁸ inserted between NheI and EcoRI of pTrcHis A	C
pNG076	σ³² inserted between NdeI and NotI of pET-22b(+)	C
pNG077	σ³² inserted between NheI and EcoRI of pTrcHis A	C
pNG083	σ²⁴ inserted between NcoI and NotI of pETM-11	N
pNG126	σ²⁴ in pTrcHis-TOPO	N