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Representative FACS measurement of the dissociation constant KD between 4M5.3 scFv displayed on yeast cells and FL-pc-QDs with different FL-p:K-p coverage (10:90, 50:50, 100:0 and control: FL-dextran). For each condition, a representative curve of the average FITC fluorescence signal per cell at a given concentration of FL-pc-QD is shown. The average fitted value of the dissociation constant for this condition is indicated above each curve.
