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. 2011 May 1;22(9):1421–1429. doi: 10.1091/mbc.E10-11-0918

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© 2011 Boyd et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — ChR1 photoreceptor localization pattern is altered in eyeless mutants. Combined immunofluorescence micrographs stained with antibodies against ChR1 (magenta) and acetylated α-tubulin (AcTub) (green). (A) A wild-type Chlamydomonas cell showing a single discrete patch of ChR1 near the end of the acetylated track of the D4 microtubule rootlet. (B) An eye2 cell showing ChR1 staining in multiple patches along the rootlet. (C) Photoreceptor localization in an individual cell of the eyeless mutant eye3–1. ChR1 is observed in multiple patches along the rootlet. (D) An eye3 cell showing ChR1 in a stripe associated with the rootlet (arrow). Discrete ChR1 patches not associated with the rootlet are also observed (arrowheads). Bars, 5 μm.