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. 2011 May 1;22(9):1421–1429. doi: 10.1091/mbc.E10-11-0918

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© 2011 Boyd et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — EYE2 localizes to a region corresponding to the chloroplast envelope in the eyespot. (A) Immunofluorescence micrographs of wild-type (left) and eye2–2 cells (right) stained with antibody directed against EYE2. EYE2 localizes to the eyespot (arrow) in wild-type cells, whereas staining is absent in the insertion mutant. (B) Combined immunofluorescence micrograph of wild-type C. reinhardtii cell stained with antibodies against EYE3 (blue), EYE2 (green), and ChR1 (red). Inset shows a close-up view of the eyespot area. EYE2 staining appears between the pigment granule layers and the plasma membrane–localized photoreceptor patch. Bars, 5 μm unless otherwise noted.