FIGURE 5:

Some monopolar attachments can be corrected independently of the CPC. (A) Chromosome IV was marked with a TetO/TetR-GFP array. The percentage of cells with bioriented chromosome IV (as judged by the localization of the GFP dot in relation to the marked SPBs) among all large-budded cells was assessed after release from nocodazole. All strains carry a genomic ipl1-321 allele as well as a multicopy plasmid with either the functional IPL1 gene (black diamonds), SGO1 (light circles), or marker only (gray squares). (B) Progression through anaphase after release from nocodazole is accelerated in cells lacking functional IPL1, as evidenced by the decrease of the percentage of large-budded cells in the population (full lines). These strains also accumulate bibudded cells at higher levels than wild-type (dotted lines). Overexpression of SGO1 did not alter the cell-cycle progression of ipl1-321 mutants, even though it affected biorientation (A). The experiments were performed at 35°C.