TABLE 1:
Plasmids and strains used
| Name | Backbone/Background | Type | Relevant markers/Strain genotype | Origin |
|---|---|---|---|---|
| BZ242 | pRS316 | CEN | URA3, SGO1 | This work |
| pBS199 | pRS316 | CEN | URA3, BUB1 | A. Murray |
| BZ11 | pRS425 | 2 μm | LEU2, SGO1 | This work |
| BZ9 | pRS425 | 2 μm | LEU2, BIR1 | This work |
| BZ40 | pRS425 | 2 μm | LEU2, SLI15 | This work |
| pTR168 | pRS315 | 2 μm | LEU2, BUB1 | A. Hoyt |
| BZ278 | pRS425 | 2 μm | LEU2, IPL1 | This work |
| T431 | N/A | CEN | pGAL1-10-CEN4, TetOx112, RS-ARS-RS, CEN4 | T. Tanaka |
| BZ277 | pRS425 | 2 μm | LEU2, MPS1 | This work |
| PB2303 | pRS316 | CEN | URA3, MPS1 | This work |
| BZ279 | pRS425 | 2 μm | LEU2, BIR1W901A | This work/K. Kaplan |
| BZ280 | pRS425 | 2 μm | LEU2, BIR1A931E, I935E | This work/K. Kaplan |
| BJ25 | pRS425 | 2 μm | LEU2, BIR1ΔN | This work |
| BZ299 | pRS425 | 2 μm | LEU2, SGO1-eGFP | This work |
| YZ669 | S288C | 1N | MATa CEN4-tetO::HIS3 TetR-GFP-LEU2 | Storchová et al., 2006 |
| YZ855 | S288C | 1N | MATa sli15-3 | This work |
| YZ1063 | S288C | 1N | MATa ipl1-321, SPC42-GFP-HIS3; pMET-rec; TetR-GFP | Dewar et al., 2004 |
| YZ1064 | S288C | 1N | MATa SPC42-GFP-HIS3; pMET-rec; TetR-GFP | Dewar et al., 2004 |
| YZ640 | S288C | 4N | MATa/a/0/a CEN4 tetO::HIS3; TetR-GFP-LEU2/TetRG- FP-LEU2/leu2/leu2 sgo1/sgo1/sgo1/sgo1; SPC29-mRFP/SPC29-mRFP/SPC29/SPC29 | This work |
| YZ997 | S288C | 4N | MATa/a/a/a bub1/bub1/bub1/bub1, CEN4- tetO::HIS3 TetR-GFP-LEU2/TetR-GFP-LEU2/leu2/leu2, SPC29-mRFP/SPC29-mRFP/SPC29/SPC29 | This work |
| YZ841 | S288C | 2N | MATa/a SLI15-GFP-HIS3/SLI15, SPC29-mRFP/SPC29 | This work |
| NT745 | S288C | 2N | MATa/a SLI15-GFP-HIS3/SLI15; SPC29-mRFP/SPC29; sgo1/sgo1 | This work |
| NT712 | S288C | 2N | MATa/a BIR1-GFP-HIS3/BIR1; SPC29-mRFP/SPC29 | This work |
| NT713 | S288C | 2N | MATa/a BIR1-GFP-HIS3/BIR1; SPC29-mRFP/SPC29; sgo1/sgo1 | This work |
| NT571 | S288C | 2N | MATa/a IPL1-GFP-HIS3/IPL1; SPC29-mRFP/SPC29 | This work |
| NT608 | S288C | 2N | MATa/a IPL1-GFP-HIS3/IPL1; SPC29-mRFP/SPC29; sgo1/sgo1 | This work |
| YZ728 | S288C | 1N | MATa SLI15-TAP-HIS3 | Ghaemmaghami et al., 2003 |
| YZ741 | S288C | 1N | MATa SLI15-TAP-HIS3; bub1::kanMX | This work |
| YZ725 | S288C | 1N | MATa NDC10-TAP-HIS3 | Ghaemmaghami et al., 2003 |
| YZ735 | S288C | 1N | MATa NDC10-TAP-HIS3; bub1::kanMX | This work |
| YZ704 | S288C | 1N | MATa YAC (URA3, TRP1, ADE2, CEN) | Storchová et al., 2006 |
| YZ406 | S288C | 1N | MATa bub1::kanMX; YAC (URA3, TRP1, ADE2, CEN) | This work |
| YZ1013 | S288C | 1N | MATa sgo1::kanMX, SPC29-mRFP; pMET-rec; TetR-GFP | This work |
| JF98 | W303 | 1N | MATa bub1delK::hph | K. Hardwick |
| YZ1071 | W303 | 1N | MATa bub1delK::hph; cdc55::kanR | This work |
| YZ1003 | W303 | 1N | MATa bub1delK::hph SGO1-GFP-HIS3, SPC29-mRFP | This work |
| YZ986 | W303 | 1N | MATa bub1::kanMX; DAM1-9myc-TRP1 | This work |
| YZ1101 | W303 | 1N | MATa ipl1-321::natMX; DAM1-9myc-TRP1 | This work |
| YZ990 | W303 | 1N | MATa DAM1-9myc-TRP1 | This work |
| YZ535 | S288C | 1N | MATa IPL1-TAP-HIS3 | This work |
| YZ808 | S288C | 1N | MATa bub1::kanMXIPL1-TAP-HIS3 | This work |
| YZ991 | S288C | 1N | MATa sgo1::hph IPL1-TAP-HIS3 | This work |
| 3108 | W303 | 1N | MATa mps1-as cdc34-2 | Mark Winey |
| YZ1056 | W303 | 1N | MATa mps1-as cdc34-2 SGO1-GFP-HIS3, SPC29-mRFP | This work |
| YZ1182 | W303 | 1N | MATa mps1-as BUB1-GFP-HIS3, SPC29-mRFP | This work |
| YZ650 | S288C | 1N | MATa ipl1-321::kanMX; CEN4-tetO::HIS3, TetR-GFP-LEU2 Spc29-mRFP | |
| YZ1174 | W303 | 1N | MATa mps1-as SLI15-GFP-HIS3, SPC29-mRFP | This work |