FIGURE 4:

The integrity of the Src–FAK–Dyn2 complex is essential for the turnover of FAs. (A–D′) IF images of FAK^−/− cells that were transfected with WT or mutant GFP-FAK as indicated. At 48 h after transfection, cells were subjected to a standard FA disassembly assay by treatment with 10 μM nocodazole for 3.5 h to disrupt MTs followed by a 1-h drug-free rinse, during which MTs repolymerize and promote FA disassembly. Cells were then fixed and stained for vinculin to label FAs. Reexpression of WT FAK (A, A′) promotes FA disassembly compared with expression of the FAK mutants (B–D′) that are unable to form the Src–FAK–Dyn2 complex. Transfected cells are indicated by asterisks (*). (E) Quantification of the percentage of cells that retained FAs following the FA disassembly assay. Results represent the average of three independent experiments.