Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 May 1;22(9):1575–1584. doi: 10.1091/mbc.E10-08-0716

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 Liu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,“ “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 3: — Genetic interactions of roy1, ypt52, and vps21 on cell growth and intracellular trafficking. (A) The indicated yeast cells were grown to exponential phase in YPD medium. Cells were harvested and resuspended in YPD medium to an OD₆₀₀ of 0.4. Three microliters of 10-fold serial dilutions were spotted onto YPD plates, incubated for 36 h at the indicated temperature, and photographed. (B) The indicated yeast cells were stained with FM2-10 as described in Materials and Methods and analyzed by fluorescence microscopy. Bar, 2 μm. (C and D) The indicated yeast cells were harvested and lysed by the TCA lysis method. Extracts were subjected to IB with the indicated antibodies. (E) The growth of the yeast strains was compared as described in A in the presence of the indicated concentration of monensin at 30°C for 36 h.