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. 2011 Apr 29;6(4):e18287. doi: 10.1371/journal.pone.0018287

Figure 1. Kinetics of Aa or S. sanguis entry and adhesion to OBA9.

Figure 1

(A and B) Aa or S. sanguis growing in middle of logarithmic phase in broth culture was applied to confluent OBA9 cells in 96-well plates (5.5×105 bacteria/100 µl/well). The multiplicity of infection (MOI) value used in these entry and adhesion assays were about 55 (5.5×105 bacteria/1.0×104 epithelial cells/well). The co-culture of bacteria and OBA9 cells was incubated at various times, and bacterial entry and attachment to OBA9 cells were evaluated by the protocol described in Materials and Methods. The number of bacteria which entered (A) or adhered (B) to OBA9 was expressed as a colony forming unit (CFU). Each point represents mean ± SD of CFU from three wells.