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. 2011 Apr 29;6(4):e18081. doi: 10.1371/journal.pone.0018081

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Sandersius et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A: stage EG XII, prior to streak formation; B: stage HH2, lateral to the streak; C: stage HH2, within the streak region. The embryo in image A is stained with rhodamine phalloidin to visualize the F actin cortex, while the embryos shown in B and C are stained with an antibody against the apically localized tight junction marker ZO-1. The areas shown are 375375 in A, and 187.5187.5 in B and C. The scale bars represent 50 in all three panels.

Inline graphic — A: stage EG XII, prior to streak formation; B: stage HH2, lateral to the streak; C: stage HH2, within the streak region. The embryo in image A is stained with rhodamine phalloidin to visualize the F actin cortex, while the embryos shown in B and C are stained with an antibody against the apically localized tight junction marker ZO-1. The areas shown are 375375 in A, and 187.5187.5 in B and C. The scale bars represent 50 in all three panels.