Figure 3. Tomographic reconstruction of wild type and transgenic C. elegans allows detailed analysis of anatomical features and fluorescence expression patterns.

(a–c) Maximum intensity projections of a transgenic animal expressing GFP in mechanosensory neurons (see also Video S1 ). The three viewpoints, sagittal (a), transversal (b) and coronal (c) reveal the circuit of the 6 labeled mechanosensory neurons (ALM_L/R, AVM, PLM_L/R, PVM). (d) Assembled single slices of a 3D reconstruction of brightfield data, displaying the anatomy of C. elegans. Combined sagittal, transversal and coronal views allow visualization of structures such as intestine, pharynx, gonad and eggs. (e) Merged, brightfield and fluorescence tomographic reconstruction of the anterior part of C. elegans. Pharyngeal muscles expressing GFP are shown in green (see also Video S2 ). Size bar indicates 50 µm.