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. 2011 Apr 29;6(4):e18887. doi: 10.1371/journal.pone.0018887

Figure 3. Detection of the regions of interest from the segmentation results and fitting to the physical models.

Figure 3

(a) Approach curve: both vertical lines correspond to the beginning (Inline graphic) and end (Inline graphic) of the electrostatic regime. (b) Approach curve, Hertz and Hooke regimes. The red vertical line and red arrow correspond to the positions Inline graphic and Inline graphic which are found by the optimization algorithm. The black line in between marks the Inline graphic-value Inline graphic for Inline graphic (beginning of Hertzian regime). (c) Retraction curve: detection of the regions of interest (decreasing regions of the piecewise affine signal). (d) Fitting of the data to the FJC model in each region of interest. No fitting is performed in the first 100 nm after the contact point Inline graphic. For each region, the extrapolation of the FJC model outside the current region of interest is shown in green.