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. 2011 Apr 29;6(4):e19064. doi: 10.1371/journal.pone.0019064

Figure 4. Effect of liposome composition on the efficiency of CT 4-induced leakage (%L) of fluorescent dye.

Figure 4

A - CT 4 (Naja kaouthia)-induced release of calcein from POPC liposomes containing different anionic lipids: pure POPC, POPC/PS5%, and POPC/SGC5%. B - Kinetics of 6-CF fluorescence during the first minutes after CT 4 addition. For these measurements, the experimental conditions (buffer composition, fluorescent dye and lipid/protein concentrations in the measured volume) correspond to those in [7]. Stationary level of CT A3 (Naja atra)-induced leakage of 6-CF demonstrated in [7] is shown as (– .. –). C - Dependence of the CT-induced leakage efficiency on the PS content (in %) in POPC liposomes. Leakage values are calculated and averaged over 2–3 measurements.