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. 2011 Apr 29;6(4):e19087. doi: 10.1371/journal.pone.0019087

Figure 4. Dependence of E. coli-mediated RNAi on Sr-CI and Eater.

Figure 4

(A) Inhibition of phagocytosis of E. coli after RNAi treatment for either EGFP (control) or Sr-CI and Eater. (B) Inhibition of phagocytosis impairs RNAi mediated by dsRNA-expressing E. coli but not by naked dsRNA. Western blot for Amph and β-tubulin from cultures of S2 cells pretreated with RNAi for either EGFP (control) or Sr-CI and Eater, and subsequently treated with either naked amph dsRNA or E. coli that express amph dsRNA. Bar graph shows levels of amphiphysin protein relative to β-tubulin. In all panels, error bars indicate standard error of the mean. * P<0.05; ** P<0.01; *** P<0.005; n.s. P>0.05; two-tailed t-test. All quantitative data are based on at least three independent experiments.