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. 2011 Apr 29;6(4):e19054. doi: 10.1371/journal.pone.0019054

Figure 2. A. Nucmer alignement of putative invasin gene from KIM 10+ and KIM D27.

Figure 2

Regions of homology are depicted as diagonal lines. The 289 bp repeat aligns with itself numerous times and results in the square pattern. B. PCR amplification of the invasin gene from KIM 10+ and KIM D27. The chromosomal region 4228610 – 4234900 was amplified from genomic DNA from either strain. The predicted 6,290 bp product was observed as the major product from KIM D27 (lanes 1 and 2), but a ladder effect was observed when KIM 10+ DNA was used (lanes 3 and 4).