Figure 1. Effect of nocodazole on hESC morphology.

(A) Cells were treated with nocodazole 50 ng/ml, or with DMSO for 24 h or left untreated. After nocodazole treatment, cells were washed with medium and grown in the presence or in the absence of Y-27632 (20 µM) for further 24 h. (B) Number of adherent cells after nocodazole treatment compared to DMSO-treated cells and untreated cells. Scale bar = 1000 µm (C) Proliferating cells of adherent cells estimated by using DAPI staining.