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. 2011 Apr 29;6(4):e19148. doi: 10.1371/journal.pone.0019148

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Yan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Amplification curves derived from three indicated recombinant PLL3.7-miR vectors. (B) Biological activity of viral miRNAs examined by dual-luciferase reporter assay. The sensor plasmid bearing four repeats complementary to each miRNA in its 3′ UTR of Renilla reporter gene was co-transfected into FHM cells, along with the indicated miRNA-expressing vector. The cells co-transfected by pLL3.7-SV40-miR-S1 and the corresponding sensor plasmid were used as positive controls. After transfection for 48 h, samples were harvested and dual luciferase activities were validated. Data are expressed as a ratio to negative controls with SDs, and the significant differences are indicated with * at p<0.05 or ** at p<0.01.