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. 2011 Apr 29;6(4):e19200. doi: 10.1371/journal.pone.0019200

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Jacobson et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Shown is an ELISA measuring total IgG (all subclasses), directed against murine thyroglobulin (Tg). The results demonstrate a significant anti-Tg response in mice immunized with hTg cDNA. For this analysis, we compared 2 non-immunized mice, the 2 best responders, out of a group of 6 that were immunized with hTg without electroporation, and 6 hTg cDNA immunized/electroporated mice. Relative to directly immunized mice, the use of an electric current (6 pulses total, 60 V/cm, and 50 ms pulse length, with a 200 ms respite between pulses) caused a significant increase in the level of anti-Tg antibodies (p = 0.037). All readings were performed in triplicate. Data are shown as mean + standard error of the mean (SEM). Animals immunized with empty vector (pCDNA3.1+) exhibited no response (data not shown) to mouse Tg.