Figure 3. Th1 nature of the Cytokine Profile.
Splenocytes were isolated from immunized/electroporated (2–3 months after the final boost) and non-immunized mice. 2×105 cells were challenged with 0, 5, or 20 µg/ml of human thyroglobulin. Supernatants were collected and were analyzed for the presence of cytokines. hTg-treated splenocytes from immunized/electroporated animals secreted significantly higher levels of interferon-gamma than controls. Mice immunized with empty vector showed no differences compared to the non-immunized controls (data not shown). All readings were performed in triplicate. Data are shown as mean + SEM.