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. 2011 Apr 29;6(4):e19251. doi: 10.1371/journal.pone.0019251

Figure 4. Akt phosphorylation suppressed Hand1 activation of a luciferase reporter gene.

Figure 4

A. Structure of Th1-luciferase reporter. Th1 represents the Th1-box sequence (CGTCTG, D-box) that is bound by Hand1. αCA is α-cardiac actin. Six successive Th1-boxes are located upstream of αCA promoter. B. Hand1-WT and –AA (T107AS109A) activate the luciferase reporter gene, whilst –DD (T107DS109D) represses this activation. Akt also inhibits Hand1-WT activation of the reporter gene. C. Hand1-DD suppresses reporter gene activation. D. Effects of three Hand1 proteins on reporter gene expression. E. Akt action on Hand1 regulation of reporter gene expression. These assays were performed with lysates from HEK293 cells transfected with corresponding plasmids. * P<0.05; ** P<0.01.